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software bionumerics 2d v6.6  (Applied Maths)

 
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    Applied Maths software bionumerics 2d v6.6
    Software Bionumerics 2d V6.6, supplied by Applied Maths, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bionumerics+2d+software/bionumerics+software/pm36012279-127-11-14
    Average 90 stars, based on 1 article reviews
    software bionumerics 2d v6.6 - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Searching for markers to identify angiogenic endothelial cells: a proteomic approach.
    Article Snippet: In the field of angiogenesis research considerable effort is put in the development of in vitro assays of angiogenesis to replace animal experiments.. Unfortunately, reproducibility of these assays frequently fails depending on the particular batch of endothelial cells delivered by the distributor.. This is due to the lack of reliable markers for the identification and isolation of angiogenic microvascular endothelial cells that have the capacity to perform all stages of the angiogenic cascade.

    Article Title: Managing prion disease risk
    Article Snippet: .. Images were compared by means of the Bionumerics 2D software (Applied Maths), assessing the presence or absence of spots and intensity variations, between paired samples and between the two groups of animals. ..



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    OMP profiles of AIEC strains. Two-dimensional polyacrylamide gel electrophoresis <t>(2D-PAGE)</t> OMP profiles of the AIEC strains grown in DMEM (2% bile salts) at 37 °C overnight: ( A ) 4I01, ( B ) 4C01, ( C ) 5I01, ( D ) 6I09, ( E ) 9C01, ( F ) 10I01, and ( G ) 18I08. Twelve percent polyacrylamide gels (13 cm; pH range: 4–7) were stained with Coomassie blue G-250. The scale bars on the left indicate molecular weights in kDa. Proteins with a well-known 2D-PAGE pattern are indicated (OmpA, OmpC, and Dps). ( H ) A representative AIEC OMP profile (blue spots) involving all the proteins present in at least 70% of the strains was superimposed on the commensal E. coli HS protein profile (orange spots). This analysis was performed using <t>BioNumerics</t> 2D software (v6.6). A total of 24 protein spots expressed differentially were selected and identified with MALDI-TOF/TOF mass spectrometry. These spots were absent in the HS OMP profile and highly frequent in the AIEC OMP profiles, as follows: 7/7 (100%, enclosed in green), 6/7 (86%, enclosed in purple), and 5/7 (71%, enclosed in red). * Spots 23 and 24 had no significant e-values in the MALDI-TOF/TOF analysis and were therefore discarded.
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    OMP profiles of AIEC strains. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) OMP profiles of the AIEC strains grown in DMEM (2% bile salts) at 37 °C overnight: ( A ) 4I01, ( B ) 4C01, ( C ) 5I01, ( D ) 6I09, ( E ) 9C01, ( F ) 10I01, and ( G ) 18I08. Twelve percent polyacrylamide gels (13 cm; pH range: 4–7) were stained with Coomassie blue G-250. The scale bars on the left indicate molecular weights in kDa. Proteins with a well-known 2D-PAGE pattern are indicated (OmpA, OmpC, and Dps). ( H ) A representative AIEC OMP profile (blue spots) involving all the proteins present in at least 70% of the strains was superimposed on the commensal E. coli HS protein profile (orange spots). This analysis was performed using BioNumerics 2D software (v6.6). A total of 24 protein spots expressed differentially were selected and identified with MALDI-TOF/TOF mass spectrometry. These spots were absent in the HS OMP profile and highly frequent in the AIEC OMP profiles, as follows: 7/7 (100%, enclosed in green), 6/7 (86%, enclosed in purple), and 5/7 (71%, enclosed in red). * Spots 23 and 24 had no significant e-values in the MALDI-TOF/TOF analysis and were therefore discarded.

    Journal: International Journal of Molecular Sciences

    Article Title: Characterization of Adherent-Invasive Escherichia coli (AIEC) Outer Membrane Proteins Provides Potential Molecular Markers to Screen Putative AIEC Strains

    doi: 10.3390/ijms23169005

    Figure Lengend Snippet: OMP profiles of AIEC strains. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) OMP profiles of the AIEC strains grown in DMEM (2% bile salts) at 37 °C overnight: ( A ) 4I01, ( B ) 4C01, ( C ) 5I01, ( D ) 6I09, ( E ) 9C01, ( F ) 10I01, and ( G ) 18I08. Twelve percent polyacrylamide gels (13 cm; pH range: 4–7) were stained with Coomassie blue G-250. The scale bars on the left indicate molecular weights in kDa. Proteins with a well-known 2D-PAGE pattern are indicated (OmpA, OmpC, and Dps). ( H ) A representative AIEC OMP profile (blue spots) involving all the proteins present in at least 70% of the strains was superimposed on the commensal E. coli HS protein profile (orange spots). This analysis was performed using BioNumerics 2D software (v6.6). A total of 24 protein spots expressed differentially were selected and identified with MALDI-TOF/TOF mass spectrometry. These spots were absent in the HS OMP profile and highly frequent in the AIEC OMP profiles, as follows: 7/7 (100%, enclosed in green), 6/7 (86%, enclosed in purple), and 5/7 (71%, enclosed in red). * Spots 23 and 24 had no significant e-values in the MALDI-TOF/TOF analysis and were therefore discarded.

    Article Snippet: Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) gels were analyzed using the software BioNumerics 2D (v6.6; Applied-Maths) as follows: The representative AIEC OMP 2D-PAGE profile was generated using the “ Matching gel 2D type ” tool, which can be used to combine gel images, using the molecular masses and isoelectric point (pI) values as a reference.

    Techniques: Polyacrylamide Gel Electrophoresis, Staining, Software, Mass Spectrometry